Directed Electronics equalizer 2500 User's Guide download pdf (Page 90)

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Perform a Rapid Run

Part # 15035786 Rev. D

• Select Custom for a partial kit or multiple 50-cycle kits. In the Cycles Remaining

field, enter the number of SBS cycles that reagents are expected to last.

NOTE

For partial kits, the software counts down the number of cycles entered. When the

cycles are low, the software prompts you to load fresh reagents.

4 Select Next.

Review Screen

1 Review the run parameters on the Review screen.

2 Select Next to proceed or select Back to change parameters.

1 2 ... 85 86 87 88 89 90 91 92 93 94 95 ... 153 154

2500 System 1
User Guide 1
Revision History 7
Table of Contents 9
Chapter 7 Troubleshooting 127 10
Index 139 10
Technical Assistance 143 10
Overview 11
HiSeq 2500 Components 13
HiSeq 2500 Software 17
Sample Sheet Overview 23
Sequencing Consumables 25
Perform a HiSeq v4 Run 27
HiSeq v4 Sequencing Workflow 29
Enter Run Parameters 31
Load and Prime Reagents 37
Perform a TruSeq v3 Run 51
TruSeq v3 Sequencing Workflow 53
Prepare Reagents for Read 2 75
Load Reagents for Read 2 77
Introduction 81
Pre-Run Volume Check 85
Perform a Rapid Run 100
Load a Flow Cell 101
Post-Run Procedures 107
Unload and Weigh Reagents 109
Perform a Maintenance Wash 111
Perform a Water Wash 115
Switch Sequencing Modes 117
Shut Down the Instrument 119
Part # 15035786 Rev. D 120
Real-Time Analysis 121
Real-Time Analysis Overview 123
Monitor Run Metrics 125
Real-Time Analysis Workflow 127
Sequencing Output Files 131
Output Folder Structure 133
Tile Numbering 135
Troubleshooting 137
Possible Run Setup Problems 139
Perform a Fluidics Check 141
Stop and Resume a Run 143
Primer Rehybridization 147
HiSeq 2500 System User Guide 149
Technical Assistance 153

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Directed Electronics equalizer 2500 User's Guide Page 90

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