Directed Electronics 7111A Specifications

PN 718113AFJanuary 2014Instructions for UseLaser Induced Fluorescence (LIF) Detector ManualLIF Instructions for UseBeckman Coulter, Inc.250 S. Kraemer

xContents Preparation,17Configuring 32 Karat for LIF Operation,18Direct Control,22Laser Control,22Time Program,23Dynamic Range,23Calibration Correcti

xiIllustrations Illustrations1 Instrument with LIF Detector,32 488 nm Laser Module Back Panel,53 LIF Optical System (Rotated 90° counterclockwise),74

xiiTables Tables1 LIF Detection Specifications,1

PN 718113AF1Laser Induced Fluorescence DetectionSpecificationsTable 1 LIF Detection SpecificationsSpecification ValueRelative Fluorescence Units (RFU

PN 718113AF2Laser Induced Fluorescence DetectionSystem Overview — LIF System System Overview — LIF SystemThe LIF system consists of an instrument, al

PN 718113AF3Laser Induced Fluorescence DetectionSystem Overview — LIF System Figure 1 Instrument with LIF DetectorThe Laser ModulesThe following sec

PN 718113AF4Laser Induced Fluorescence DetectionSystem Overview — LIF System WARNINGDuring normal operation of the LIF Detector, laser light is not a

PN 718113AF5Laser Induced Fluorescence DetectionSystem Overview — LIF System Figure 2 488 nm Laser Module Back Panel635 nm Laser Module IMPORTANT Th

PN 718113AF6Laser Induced Fluorescence DetectionSystem Overview — LIF System connected to your laser’s interlock circuit. The connectors and switches

PN 718113AF7Laser Induced Fluorescence DetectionSystem Overview — LIF System Figure 3 LIF Optical System (Rotated 90° counterclockwise)1. Laser 12.

Laser Induced Fluorescence (LIF) Detector ManualLIF Instructions for UsePN 718113AF (January 2014)© 2012-2014 Beckman Coulter, Inc.All rights reserved

PN 718113AF8Laser Induced Fluorescence DetectionInstallation Figure 4 Optical DiagramInstallationWARNINGBe sure to power down the instrument before

PN 718113AF9Laser Induced Fluorescence DetectionInstallation Bench SpaceThe instrument and the laser require an area of approximately four feet by tw

PN 718113AF10Laser Induced Fluorescence DetectionInstallation Figure 5 Installing the Interconnect Module1. Probe Holder2. Fiber Optic Connector3. I

PN 718113AF11Laser Induced Fluorescence DetectionInstallation Installing the LIF Detector ModulePrior to installing the LIF Detector, install the fil

PN 718113AF12Laser Induced Fluorescence DetectionInstallation 6If the filter(s) are removed from a channel, re-install the beam blocker in its place

PN 718113AF13Laser Induced Fluorescence DetectionInstallation Installing the Filter Housing into the DetectorInsert the filter housing into the openi

PN 718113AF14Laser Induced Fluorescence DetectionInstallation Installing the LIF Detector into the InstrumentWARNINGBe sure to power down the instrum

PN 718113AF15Laser Induced Fluorescence DetectionInstallation Figure 8 Installing the LIF Plug and Probe StabilizerInstall the cartridge containing

PN 718113AF16Laser Induced Fluorescence DetectionInstallation Figure 9 Connecting the Probe to the LIF Cartridge1. Probe Stabilizer2. Probe Clamp190

PN 718113AF17Laser Induced Fluorescence DetectionOperation OperationPreparationWARNINGMake sure the laser fiber optic cable and interlock devices are

PN 718113AFiiiRevision HistoryThis document applies to the latest software listed and higher versions. When a subsequent software version changes the

PN 718113AF18Laser Induced Fluorescence DetectionOperation Configuring 32 Karat for LIF Operation 1The 32 Karat Enterprise screen is shown in Figure

PN 718113AF19Laser Induced Fluorescence DetectionOperation 2Name the new instrument. Right-click the newly created instrument to configure, then clic

PN 718113AF20Laser Induced Fluorescence DetectionOperation 3Choose the type of instrument you want to control, P/ACE MDQ CE or ProteomeLab PA 800. A

PN 718113AF21Laser Induced Fluorescence DetectionOperation The P/ACE System MDQ Instrument Configuration screen allows the user to define the types o

PN 718113AF22Laser Induced Fluorescence DetectionOperation Direct ControlFigure 16 shows the Direct Control screen for instruments configured with an

PN 718113AF23Laser Induced Fluorescence DetectionOperation status on the Direct Control and Status screen reflects the position of this on/off switch

PN 718113AF24Laser Induced Fluorescence DetectionOperation Figure 19 Electropherograms (A, B) with different Dynamic RangesFor optimal results, sele

PN 718113AF25Laser Induced Fluorescence DetectionOperation • Fluorescence yield of the molecule being measured • Background buffer composition and p

PN 718113AF26Laser Induced Fluorescence DetectionOperation Once the CCF value is calculated and accepted by the user, all subsequent measurements wil

PN 718113AF27Laser Induced Fluorescence DetectionOperation 4Click on the LIF Calibration Wizard. See Figure 20.Figure 20 Accessing the LIF Calibrati

PN 718113AFivRevision History

PN 718113AF28Laser Induced Fluorescence DetectionOperation Figure 21 Calibration Wizard – Step 1Selecting Auto Mode will guide the user through a se

PN 718113AF29Laser Induced Fluorescence DetectionOperation 2Click Next.Figure 22 Calibration Wizard – Step 2

PN 718113AF30Laser Induced Fluorescence DetectionOperation Calibration Wizard – Step 3The following Calibration Wizard – Step 3 screen appears, as sh

PN 718113AF31Laser Induced Fluorescence DetectionOperation 2After installing the vials in the tray, close the instrument lid and click Next.The scree

PN 718113AF32Laser Induced Fluorescence DetectionCare and Maintenance Manual ModeThe Manual Mode allows the user to enter values for the Calibration

PN 718113AF33Laser Induced Fluorescence DetectionCare and Maintenance CleaningClean the exterior surfaces of the detector and laser module regularly

© 2014 Beckman Coulter, Inc.All Rights Reservedwww.beckmancoulter.com

PN 718113AFvSafety NoticeRead all product manuals and consult with Beckman Coulter-trained personnel before attempting to operate instrument. Do not a

PN 718113AFviSafety NoticeLaser Light Laser LightThis symbol indicates that a potential hazard to personal safety exists from a laser source. When th

PN 718113AFviiSafety NoticeFCC Class A Compliance CAUTIONDo not attempt to load or remove labware manually while the instrument is in operation.WARNI

PN 718113AFviiiSafety NoticeEMI Compliance

ixContentsRevision History,iiiSafety Notice,vAlerts for Danger, Warning, Caution, Important, and Note,vHigh Voltage Danger,vLaser Light,viLaser Specif